Melamine Diagnostic ELISA Kit for Fresh Milk 96 Wells/Kit Sensitivity 0.5
1. Melamine Diagnostic ELISA Kit Principle
This kit adopts the indirect competitive ELISA method. The conjugated antigen is pre-coated on the microwell strip. The residual melamine in the sample will compete with the conjugated antigen pre-coated on the microwell strip for anti-melamine antibody. After adding the enzyme marker , using TMB substrate for color development, the absorbance value of the sample is negatively correlated with the content of the residual melamine contained in it, and the content of the corresponding residual melamine can be obtained by comparing it with the standard curve.
2. Technical specifications
Sensitivity: 0.5ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Butter, candy, cheese 10ppb
Cross-reaction rate:
Melamine 100%
Recovery rate:
Butter 90±25%
Candy, cheese 95±25%
3. Melamine Diagnostic ELISA Kit Components
| 1 | Micro-well strips | 12 strips with 8 removable wells each |
| 2 | 6× standard solution (1 mL each) | 0ppb | 0.5ppb |
| 1.5ppb | 4.5ppb |
| 13.5ppb | 40.5ppb |
| 3 | Spiking standard solution | 1 ppm | 1 ml |
| 4 | Enzyme conjugate | 7ml | red cap |
| 5 | Antibody working solution | 7ml | blue cap |
| 6 | Substrate A | 7ml | white cap |
| 7 | SubstrateB | 7ml | black cap |
| 8 | Stop solution | 7ml | yellow cap |
| 9 | 10x sample extracting A solution | 50ml | transparent cap |
| 10 | 20x sample extracting B solution | 50ml | blue cap |
| 11 | 20× concentrated washing buffer | 15ml | white cap |
4. Sample Preparation
Instructions for use (the following points must be dealt with before preprocessing)
1) The experiment can only use disposable tips, and the tips must be replaced when using different reagents;
2) Before the experiment, each experimental equipment must be cleaned and re-cleaned if necessary to avoid contamination interfering with the experimental results.
Solution preparation
1) Sample extraction solution
Dilute 10-fold sample extraction solution A with deionized water at 1:9;
2) Sample extraction B solution
Dilute the 20-fold sample extraction solution B with deionized water at 1:19.
4.1 Butter
1) Take 1g of fresh butter into a 50ml centrifuge tube, add 5ml of sample to extract A solution, incubate in a water bath at 70°C for 5min, vortex for 5min, and centrifuge at room temperature above 4000r/min for 5min;
2) Take 50ul of the lower liquid for analysis
Sample dilution factor: 5
4.2 Candy, cheese
1) Take 1g of fresh sample into a 50ml centrifuge tube, add 5ml of sample to extract B solution, incubate in a water bath at 70°C for 5min, vortex for 5min, and centrifuge at room temperature above 4000r/min for 5min;
2) Take 50ul of the lower liquid for analysis
Sample dilution factor: 5
5. Storage and expiry date
Storage: stored at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)
Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.
Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.
Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.
Q5: What is the payment method?
A5: We receive payment by T/T.
Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.
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96 Wells/Kit Melamine Rapid Diagnostic ELISA Kit For Fresh Milk Sensitivity 0.5 Images
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